使用方法【源自文獻(xiàn)，僅作參考】

文獻(xiàn)1，Yan J et al. The bifunctional autophagic flux by 2-deoxyglucose to control survival or growth of prostate cancer cells. Cancer Res. 2005 Aug 1;65(15):7023-30. PMID: 16061689

體外研究（細(xì)胞實(shí)驗(yàn)）：

細(xì)胞類型（Cell types）：MCF-7 cells

實(shí)驗(yàn)方法（Assay）：For cell growth assay, MCF-7 cells were plated at 3 × 104 cells per well in flat-bottomed 96-well plates in 100 μL of culture medium. After 24 hours, cells were fed with fresh medium including 2-DG at doses of 0, 4, 8, or 16 mmol/L. At days 1, 3, and 5 after 2-DG exposure, cells were fixed with 1% glutaraldehyde, replaced with PBS, and stored at 4°C. At the end of an experiment, all of the plates were stained with 0.02% aqueous crystal violet for 30 minutes and rinsed with deionized water. After redissolving the bound crystal violet in 70% ethanol, the absorbance was determined at 590 nm. 

實(shí)驗(yàn)結(jié)果（Result）：Cell growth was significantly inhibited by 2-DG at doses of 4, 8, or 16 mmol/L for 1, 3, or 5 days in a dose- and time-dependent manner (P < 0.001).

體內(nèi)研究（動(dòng)物模型）：

動(dòng)物模型（Animal Model）：At 21 days of age, rats were injected with 50 mg 1-methyl-1-nitrosourea per kilogram of body weight (i.p.). Six days following carcinogen injection, all rats were randomized into one of three groups, 30 rats per group, and werefed ad libitum AIN-93G diet containing0.0%, 0.02%, or 0.03% (w/w) 2-DGfor 5 weeks.

實(shí)驗(yàn)結(jié)果（Result）：2-DG decreased the incidence and multiplicity of mammary carcinomas and prolonged cancer latency (P < 0.05). The 0.02% dose of 2-DG had no effect on circulating levels of glucose, insulin, insulin-like growth factor-I, IGF binding protein-3, leptin, or body weight gain.

文獻(xiàn)2，Wang H et al. 2-deoxy-D-glucose enhances anesthetic effects in mice. Anesth Analg. 2015 Feb; 120(2):312-9. PMID: 25390277

體內(nèi)研究（動(dòng)物模型）：

動(dòng)物模型（Animal Model）：Female Sprague-Dawley rats at 20 days of age

配制方法（Formulation）：2-DGwasdiluted insaline. 

實(shí)驗(yàn)方法（Assay）：2-DG (1000 mg/kg), 3-NP (50 mg/kg),30 or saline was injected [intraperitoneally (ip)] 5 minutes before isoflurane anesthesia.Idebenone (200 mg/kg) or L-carnitine (100 mg/kg) was injected 15 minutes before isoflurane anesthesia (10 minutes before treatment with 2-DG or 3-NP).

實(shí)驗(yàn)結(jié)果（Result）：2-DG enhances isoflurane-induced loss of righting reflex (LORR) in mice.