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HighYield T7 Digoxigenin RNA Labeling Kit
| 貨號 | RNT-101-DIGX | 售價(jià)（元） | 4425 |
| 規(guī)格 | 30T x 20 μl | CAS號 |
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產(chǎn)品信息
|
品牌 |
貨號 |
名稱 |
規(guī)格 |
單價(jià) |
|
Jena Bioscience |
RNT-101-DIGX |
HighYield T7 Digoxigenin RNA Labeling Kit |
30T x 20 μl |
4425.00 |
HighYield T7地高辛RNA標(biāo)記試劑盒設(shè)計(jì)用于通過體外轉(zhuǎn)錄隨機(jī)產(chǎn)生地高辛修飾的RNA探針。這種探針非常適合原位雜交和Northern Blot實(shí)驗(yàn)。標(biāo)記原則類似于DIG RNA標(biāo)記混合物（羅氏）和DIG RNA標(biāo)簽試劑盒（羅氏公司）的基本標(biāo)記原則。使用優(yōu)化的反應(yīng)緩沖液和T7 RNA標(biāo)記聚合酶混合物，DIG-11-UTP作為天然對應(yīng)物UTP的替代物有效地并入RNA。35%DIG-11-UTP替代通常會在反應(yīng)和標(biāo)記效率之間實(shí)現(xiàn)最佳平衡。然而，使用單核苷酸格式可以輕松實(shí)現(xiàn)DIG-11-UTP/UTP比值的個(gè)別優(yōu)化。由此產(chǎn)生的地高辛修飾RNA探針隨后可通過HRP或AP修飾地高辛抗體檢測。
該試劑盒含有足夠的試劑，用于30個(gè)標(biāo)記反應(yīng)，每個(gè)反應(yīng)20μl（35%DIG-11-UTP替代物，1 mM ATP，GTP，CTP，0.65 mM UTP，0.35 mM DIG-11-UTP）。
Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)
HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based
ATP - Solution
1x 100 μl (100 mM)
GTP - Solution
1x 100 μl (100 mM)
CTP - Solution
1x 100 μl (100 mM)
UTP - Solution
1x 100 μl (100 mM)
DIG-11-UTP
1x 25 μl (10 mM)
T7 G-initiating control template (1.4
kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor
resulting in approx. 1400 nt RNA transcript
PCR-grade water
1x 1.2 ml
DTT
1x 100 μl (100 mM)
To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)
|
品牌 |
貨號 |
名稱 |
規(guī)格 |
單價(jià) |
|
Jena Bioscience |
RNT-106-S |
HighYield T7 mRNA Synthesis Kit (Ψ-UTP) |
15reactions x 20 μl |
4425.00 |
|
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RNT-106-L |
HighYield T7 mRNA Synthesis Kit (Ψ-UTP) |
50reactions x 20 μl |
8925.00 |
|
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RNT-105 |
HighYield T7 sgRNA Synthesis Kit (SpCas9) |
50reactions |
4425.00 |
|
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RNT-104 |
HighYield T7 mRNA Synthesis Kit (m5CTP/Ψ-UTP) |
15reactions x 20 μl |
7875.00 |
|
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RNT-103 |
HighYield T7 ARCA mRNA Synthesis Kit (m5CTP/Ψ-UTP) |
15reactions x 20 μl |
8805.00 |
|
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RNT-102-S |
HighYield T7 ARCA mRNA Synthesis Kit |
15reactions x 20 μl |
4125.00 |
|
Jena Bioscience |
RNT-102-L |
HighYield T7 ARCA mRNA Synthesis Kit |
50reactions x 20 μl |
8175.00 |
|
Jena Bioscience |
RNT-101-FAMX |
HighYield T7 Fluorescein RNA Labeling Kit |
20reactions x 20 μl |
4875.00 |
|
Jena Bioscience |
RNT-101-DIGX |
HighYield T7 Digoxigenin RNA Labeling Kit |
30reactions x 20 μl |
4425.00 |
|
Jena Bioscience |
RNT-101-CY5 |
HighYield T7 Cy5 RNA Labeling Kit |
20reactions x 20 μl |
4875.00 |
|
Jena Bioscience |
RNT-101-CY3 |
HighYield T7 Cy3 RNA Labeling Kit |
20reactions x 20 μl |
4875.00 |
|
Jena Bioscience |
RNT-101-BIOX |
HighYield T7 Biotin11 RNA Labeling Kit |
30reactions x 20 μl |
4425.00 |
|
Jena Bioscience |
RNT-101-BIO16 |
HighYield T7 Biotin16 RNA Labeling Kit |
30reactions x 20 μl |
4425.00 |
|
Jena Bioscience |
RNT-101-AZ |
HighYield T7 Azide RNA Labeling Kit |
25reactions x 20 μl |
3975.00 |
|
Jena Bioscience |
RNT-101-AF647 |
HighYield T7 AF647 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
Jena Bioscience |
RNT-101-AF594 |
HighYield T7 AF594 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
Jena Bioscience |
RNT-101-AF555 |
HighYield T7 AF555 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
Jena Bioscience |
RNT-101-AF488 |
HighYield T7 AF488 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
Jena Bioscience |
RNT-101-488-S |
HighYield T7 Atto488 RNA Labeling Kit |
20reactions x 20 μl |
5925.00 |
|
Jena Bioscience |
RNT-101-488-L |
HighYield T7 Atto488 RNA Labeling Kit |
50reactions x 20 μl |
13755.00 |
|
Jena Bioscience |
RNT-101 |
HighYield T7 RNA Synthesis Kit |
50reactions x 20 μl |
2925.00 |