產(chǎn)品信息

      The NxSeq AmpFREE Low DNA Library Kit and Adaptors are only compatible with Illumina sequencers.Each NxSeq AmpFREE Low DNA Library Kit contains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG) and Elution Buffer (EB). Adaptors must be purchased separately.Each box of NxSeq Adaptors contains (12) different indexed Illumina-compatible adaptors with enough of each adapter for 4 library reactions. Box 1 contains adaptors 1-12 and Box 2 contains adapters 13-24.
      NxSeq AmpFREE低DNA文庫套件和適配器僅與Illumina測序儀兼容。每個NxSeq AmpFREE低DNA文庫套件包含酶混合物（EM），2X緩沖液（2XB），連接酶（LIG）和洗脫緩沖液（EB）。接頭必須單獨購買。每盒NxSeq接頭包含（12）不同的Illumina兼容 index  ，每個接頭足夠4個文庫反應(yīng)。 Box1包含接頭1-12，Box2包含接頭13-24。
優(yōu)點：
? Low Input: Requires as little as 75 ng of sheared input DNA allowing use of limiting samples.
- 低輸入：需要少至75 ng的剪切輸入DNA，允許使用限制性樣品。
? High Efficiency: Optimized adaptor ligation produces more sequenceable fragments in each library, yielding better coverage & depth from single or multiplexed libraries.
- 高效：優(yōu)化的接頭連接在每個文庫中產(chǎn)生更多可測序的片段，從單個或多重文庫中獲得更好的覆蓋率和深度。
? PCR-free: Prevents the introduction of PCR-bias, providing more uniform coverage.
- 無PCR：防止引入PCR偏向，提供更均勻的覆蓋。

數(shù)據(jù)：

圖1. Percentage of library DNA with correctly ligated adaptors measured by qPCR.

Duplicate libraries were prepped per kit/organism (Human, Staphylococcus aureus, Rhodobacter sphaeroides (1 library only), and E. coli) according to the manufacturer’s recommended input amounts and protocols. Adaptor ligation efficiency was measured by qPCR using the KAPA Library Quantification Kit (Complete ROX Low, cat #KK4873) and matching amplified library as an internal standard.

組分

Each NxSeq AmpFREE Low DNA Library Kit contains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG) and Elution Buffer (EB). Adaptors must be purchased separately.
每個NxSeq AmpFREE低DNA文庫試劑盒包含酶混合物（EM），2X緩沖液（2XB），連接酶（LIG）和洗脫緩沖液（EB）。 接頭必須單獨購買。

應(yīng)用

The NxSeq? AmpFREE Low DNA Library Kit allows you to build the best fragment DNA libraries possible. We optimized each step of the protocol to ensure peak performance on Illumina sequencers. In addition, these kits require only 75 ng of sheared DNA input, and produce libraries in about 2 hours using a streamlined, easy to follow protocol.
NxSeq?PampFREE低DNA文庫試劑盒允許您構(gòu)建最佳的片段DNA文庫。 我們優(yōu)化了操作的每個步驟，以確保Illumina測序儀的峰值性能。 此外，這些試劑盒僅需要75 ng的剪切DNA輸入，并使用簡化的易于遵循的方案，能在約2小時內(nèi)產(chǎn)生文庫。