產(chǎn)品信息

     The End-It? DNA End-Repair Kit is used to convert DNA with damaged or incompatible 5′-protruding and/or 3′-protruding ends  to 5′-phosphorylated, blunt-end DNA for fast and efficient   blunt-end ligation into plasmid, cosmid, fosmid, BAC, other cloning vectors, or nextgen DNA sequencing adaptors. End-repaired DNA can be efficiently and rapidly blunt-end ligated into DNA cloning vectors or to next-gen DNA sequencing adaptors using Lucigen’s Fast-Link? DNA Ligation Kits.
     End-It DNA末端-修復(fù)試劑盒用于將帶有受損或不相容的5，-突出和/或3，-突出末端的DNA轉(zhuǎn)化為5，-磷酸化的平末端DNA，用于隨后添加下一代DNA測(cè)序接頭，或用于克隆。用End-It試劑盒處理DNA后，可以使用Fast-Link DNA連接試劑盒（Lucigen）進(jìn)行快速有效的平末端連接.
優(yōu)點(diǎn)：
? Complete conversion of DNA into blunt-ended, phosphorylated DNA
? Repair sheared, nebulized or restriction enzyme-digested genomic DNA
? Prepare templates for NGS (next-generation sequencing) or shotgun library preparation
? 將DNA完全轉(zhuǎn)化為末端鈍化的磷酸化DNA
? 修復(fù)剪切，霧化或限制性?xún)?nèi)切酶消化的基因組DNA
? 準(zhǔn)備用于NGS（二代測(cè)序）或shot彈槍文庫(kù)準(zhǔn)備的模板
組成成分：
? End-Repair Enzyme Mix：-20°C
? End-Repair 10X Buffer：-20°C
? ATP：-20°C
? dNTPs：-20°C