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3-methylcytosine (3-mC) Antibody
| 貨號 | C15410209 | 售價（元） | 咨詢 |
| 規(guī)格 | 50ug | CAS號 |
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Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA.
| Lot | A2111-0010 |
|---|---|
| Concentration | 1.80 μg/μl |
| Species reactivity | Human, mouse, other (wide range) |
| Type | Polyclonal |
| Purity | Protein G purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Dot Blotting | 1:400 | Fig 1 |
* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.
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Validation Data
Figure 1. Dot blot analysis using the Diagenode antibody directed against 3-mC
To demonstrate the specificity of the Diagenode antibody against 3-mC (cat. No. C15410209), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.